Insulin antibody (AA 25-54)

Catalog No. ABIN1172907

Product Details anti-Insulin Antibody

Target: Insulin (INS)

Binding Specificity: AA 25-54

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Insulin (INS)

Specificity: The antibody is a rabbit polyclonal antibody raised against INS. It has been selected for its ability to recognize INS in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Insulin (INS) corresdonding to Phe25~Ala54 (Accession # P01317) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin (INS Products)

Synonyms: IDDM2 antibody, ILPR antibody, IRDN antibody, MODY10 antibody, ins1 antibody, xins antibody, ins1-a antibody, Insulin antibody, AA986540 antibody, Ins-2 antibody, InsII antibody, Mody antibody, Mody4 antibody, proinsulin antibody, zgc:109842 antibody, igf2-A antibody, ins antibody, ins-a antibody, ins-b antibody, insulin antibody, insulin precursor antibody, insulin II antibody, preproinsulin antibody, insulin L homeolog antibody, insulin S homeolog antibody, INS antibody, INS-IGF2 antibody, ins antibody, Ins antibody, PIN antibody, Ins2 antibody, ins.L antibody, ins.S antibody

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation