Caspase 1 antibody (AA 120-297)

Catalog No. ABIN1174806

Product Details anti-Caspase 1 Antibody

Target: Caspase 1 (CASP1)

Binding Specificity: AA 120-297

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Caspase 1 (CASP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against CASP1. It has been selected for its ability to recognize CASP1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Caspase 1 (CASP1) corresdonding to Asn120~Asp297 (Accession # Q9N2I1)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-Caspase 1 antibody (ABIN1174806)

Park, Liu, Thulasi Raman, Landreth, Liu, Zhou: "NS1 Protein of 2009 Pandemic Influenza A Virus Inhibits Porcine NLRP3 Inflammasome-Mediated Interleukin-1 Beta Production by Suppressing ASC Ubiquitination." in: Journal of virology, Vol. 92, Issue 8, (2018) (PubMed).

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase 1 (CASP1 Products)

Synonyms: ICE antibody, IL1BC antibody, P45 antibody, Il1bc antibody, Ice antibody, CASP1 antibody, CG5370 antibody, DCP-1 antibody, DCP1 antibody, Dcp1 antibody, Dmel\\CG5370 antibody, ccp1 antibody, dcp-1 antibody, dcp1 antibody, l(2)01862 antibody, l(2)02132 antibody, caspase 1 antibody, caspase 1, apoptosis-related cysteine peptidase antibody, caspase 4, apoptosis-related cysteine peptidase antibody, Death caspase-1 antibody, caspase-1 antibody, caspase antibody, caspase recruitment domain-containing protein 16 antibody, caspase 4 antibody, CASP1 antibody, Casp1 antibody, CASP4 antibody, Dcp-1 antibody, Caspase1 antibody, LOC5564196 antibody, LOC451519 antibody

Background: ICE, IL1BC, IL1-BC, P45, Interleukin 1 Beta Converting Enzyme, Apoptosis-Related Cysteine Peptidase, Interleukin-1 beta convertase

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome