Actin antibody

Catalog No. ABIN1720863

Product Details anti-Actin Antibody

Target: Actin

Reactivity: Barley, Arabidopsis thaliana, Zea mays, Soybean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: TAIR: At2g37620, At2g18780, At2g53750, At2g59370, At2g42100, At5g09810, At2g49240, At2g12110, At2g46520

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardtii (too high background for this species)

Predicted Reactivity: Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cucumis sativus, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Vicia faba

Purification: serum

Immunogen: ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520

Application Details

Application Notes: 1 : 2500 with standard ECL (WB), 1: 250 (IF)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Handling Advice: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-Actin antibody (ABIN1720863)

Zheng, Fang, Pan, Sun, Deng, Grosser, Guo: "iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo." in: Journal of proteome research, Vol. 13, Issue 6, pp. 2998-3015, (2014) (PubMed).

Target Details for Actin

Target: Actin

Alternative Name: Actin (ACT) (Actin Products)

Synonyms: Act57 antibody, Act57A antibody, Act57b antibody, Actin antibody, BEST:RE38067 antibody, C antibody, CG10067 antibody, CG30294 antibody, Dmel\\CG10067 antibody, M antibody, T10 antibody, act 57A antibody, act57A antibody, act57B antibody, act57b antibody, actin antibody, anon-EST:CL2c12 antibody, 143060_f_at antibody, CG7478 antibody, D antibody, Dmel\\CG7478 antibody, act 79B antibody, act79B antibody, mus79B antibody, Act(88F) antibody, Act88-F antibody, Act88f antibody, CG5178 antibody, Dmel\\CG5178 antibody, E antibody, Ifm(3)7 antibody, act 88F antibody, act88F antibody, rsd antibody, Act87e antibody, CG18290 antibody, Dmel\\CG18290 antibody, act 87E antibody, act87E antibody, mus87E antibody, Act88F antibody, Dsim\\GD19025 antibody, GD19025 antibody, dsim_GLEANR_2829 antibody, 42A antibody, A antibody, AFFX-Dros-ACTIN_M_r_at antibody, Act antibody, Act42 antibody, Act42a antibody, Actin/BAP47 antibody, BAP47 antibody, CG12051 antibody, Dmel\\CG12051 antibody, act 42A antibody, act42A antibody, cytoplasmic antibody, Actin 57B antibody, Actin 79B antibody, Actin 88F antibody, Actin 87E antibody, Actin-like protein C08B11.6 antibody, hypothetical protein antibody, ACT_COLSC Actin (ISS) antibody, Actin 42A antibody, actin gamma 1 antibody, actin antibody, Actin, gamma 1 antibody, si:ch211-241j12.3 antibody, Act57B antibody, Act79B antibody, Act88F antibody, Act87E antibody, arp-6 antibody, Dsim\Act88F antibody, ACTIN antibody, Act42A antibody, ACTG1 antibody, LOC548170 antibody, Bm1_16810 antibody, IDA5 antibody, LOC100342017 antibody, si:ch211-241j12.3 antibody

Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Molecular Weight: expected: 41.6 kDa, appearent: 45 kDa

UniProt: P0CJ46, Q96292, P0CJ47, P53494, Q8RYC2, P53492, Q96293, P53496, P53497