AGO2 antibody

Catalog No. ABIN1720882

Product Details anti-AGO2 Antibody

Target: AGO2 (Argonaute 2 (AGO2))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: TAIR: At1g48410

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays

Predicted Reactivity: Capsella rubella, Solanum tuberosum

Purification: serum

Immunogen: KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280

Application Details

Application Notes: 1: 250 - 1: 500 (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Handling Advice: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: AGO2 (AGO2 Products)

Synonyms: EIF2C2 antibody, T19E23.7 antibody, T19E23_7 antibody, argonaute 2 antibody, Q10 antibody, Eif2c2 antibody, Argonaute2 antibody, eif2c1 antibody, eif2c2 antibody, 1110029L17Rik antibody, 2310051F07Rik antibody, AI225898 antibody, AL022874 antibody, AW546247 antibody, ENSMUSG00000072493 antibody, Gerp95 antibody, Gm10365 antibody, mKIAA4215 antibody, AGO2 antibody, AG02 antibody, AGO 2 antibody, Ago-2 antibody, Ago2 antibody, CG13452 antibody, CG7439 antibody, Dm Ago2 antibody, Dmel\\CG7439 antibody, ago antibody, ago-2 antibody, ago2 antibody, dAGO2 antibody, dAgo2 antibody, eIF2C2 antibody, argonaute 2, RISC catalytic component antibody, Argonaute family protein antibody, argonaute 2, RISC catalytic component L homeolog antibody, argonaute RISC catalytic subunit 2 antibody, argonaute RISC catalytic component 2 antibody, Argonaute 2 antibody, AGO2 antibody, Ago2 antibody, ago2.L antibody, ago2 antibody

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molecular Weight: expected: 116.4 kDa, appearent: 130 kDa

UniProt: Q9SHF3

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization