Apolioprotein A5 (APOA5) antibody

Antigen: Apolioprotein A5 (APOA5)

Synonyms: RAP3, Apoav, 1300007O05Rik, APOAV, APOA-V, MGC126836, MGC126838, MGC108612, APOA5, MGC140487

Clonality: Monoclonal (2G1H11)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN173293

Additional Information

Immunogen: Source of Antigen: E. coli

Format: Protein G purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2G1H11

Description: Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. In, additions, peroxisome proliferators-activated receptor-agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Despite these compelling molecular biology data, relatively little is known about ApoA5 protein in human serum. Recently, it was demonstrated that ApoA5 is the present in the human serum detected by polyclonal antibodies against both the HN2 and COOH termini, although at much lower concentration than other apolipoproteins

Application Details

Application Notes: ELISA: 1:4000. WB: 1:1000 to 1:2000

Concentration: 1 mg/ml

Purification: Affinity chromatography on a column with immobilized protein G.

Buffer: Concentration of antibody is 1 mg/ml in 50% Glycerol in PBS, pH 7.2. AZIDE FREE.

Storage: The antibody remains stable and fully active until the expiry date when stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated and freezing thawing. Aliquot with Sodium Azide will be stable at 4ºC for 3 months.

Restrictions: For Research Use only