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NrCAM antibody (Extracellular Domain) (PE)

NRCAM Reactivity: Mouse WB, ICC, IF Host: Mouse Monoclonal S364-51 PE
Catalog No. ABIN1741303
  • Target See all NrCAM (NRCAM) Antibodies
    NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))
    Binding Specificity
    • 19
    • 10
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 30-845, Extracellular Domain
    Reactivity
    • 28
    • 23
    • 10
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    Mouse
    Host
    • 25
    • 18
    Mouse
    Clonality
    • 25
    • 18
    Monoclonal
    Conjugate
    • 23
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NrCAM antibody is conjugated to PE
    Application
    • 37
    • 20
    • 12
    • 12
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~160 kDa.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.
    Clone
    S364-51
    Isotype
    IgG2a
    Top Product
    Discover our top product NRCAM Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN1741303 was sufficient for detection of NrCAM in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))
    Alternative Name
    NrCAM (NRCAM Products)
    Synonyms
    CD56 antibody, MSK39 antibody, NCAM antibody, Bravo antibody, C030017F07Rik antibody, C130076O07Rik antibody, mKIAA0343 antibody, NRCAM antibody, si:dkey-240a12.1 antibody, Nr-CAM antibody, neural cell adhesion molecule 1 antibody, neuronal cell adhesion molecule antibody, neuronal cell adhesion molecule L homeolog antibody, neuronal cell adhesion molecule a antibody, NCAM1 antibody, NRCAM antibody, Nrcam antibody, nrcam.L antibody, nrcama antibody, nrcam antibody
    Background
    Neuronal cell adhesion molecule (NrCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NrCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NrCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NrCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NrCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NrCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NrCAM expression may play a role in the severity of certain types of tumors. NrCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NrCAM expression is upregulated in intraductal hyperplasia. Antisense NrCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NrCAM maps to chromosome 7q31.1-q31.2.
    Gene ID
    319504
    NCBI Accession
    NP_001139503
    UniProt
    Q810U4
    Pathways
    Regulation of Cell Size
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