pADPR antibody (Agarose Beads)

Catalog No. ABIN1741705

Product Details

Target: pADPR

Reactivity: All Species

Host: Mouse

Clonality: Monoclonal

Conjugate: Agarose Beads

Application: Immunoprecipitation (IP)

Characteristics: Recognizes DNA-damage and apoptosis related poly-ADP ribosylated proteins and poly (ADP-ribose) polymer

Purification: Protein A affinity column

Immunogen: poly(ADP-ribose)

Clone: 10H

Isotype: IgG3

Application Details

Application Notes: 0.5 µg/IP reaction

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4 with 0,05 % sodium azides

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C

Storage Comment: Stable for 1 year from date of shipment when stored at -20 °C or -70 °C. Stable for at least 1 month at 4 °C.

References

Kanai, Tong, Sugihara, Wang, Fukasawa, Miwa: "Involvement of poly(ADP-Ribose) polymerase 1 and poly(ADP-Ribosyl)ation in regulation of centrosome function." in: Molecular and cellular biology, Vol. 23, Issue 7, pp. 2451-62, (2003) (PubMed).

Target Details

Target: pADPR

Background: Synonyms: poly(ADP-ribose)
Poly(ADP-ribose) or pADPr is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerase (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer pADPr, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. pADPr becomes attached to nuclear proteins, and to PARP itself (automodification).