VAMP8 antibody (Cytoplasmic Domain)

Catalog No. ABIN1742195

Product Details anti-VAMP8 Antibody

Target: VAMP8 (Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8))

Binding Specificity: AA 1-74, Cytoplasmic Domain

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant cytoplasmic domain of rat endobrevin (aa 1-74).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-VAMP8 antibody (ABIN1742195)

Imbery, Bhattacharya, Khuder, Weiss, Goswamee, Iqbal, Giovannucci: "cAMP dependent recruitment of acidic organelles for Ca2+ signaling in the salivary gland." in: American journal of physiology. Cell physiology, pp. ajpcell.00010.2016, (2016) (PubMed).

Matte, Casgrain, Séguin, Moradin, Hong, Descoteaux: "Leishmania major Promastigotes Evade LC3-Associated Phagocytosis through the Action of GP63." in: PLoS pathogens, Vol. 12, Issue 6, pp. e1005690, (2016) (PubMed).

Demais, Barthélémy, Perraut, Ungerer, Keime, Reibel, Pfrieger: "Reversal of Pathologic Lipid Accumulation in NPC1-Deficient Neurons by Drug-Promoted Release of LAMP1-Coated Lamellar Inclusions." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 30, pp. 8012-25, (2016) (PubMed).

Golebiewska, Harper, Williams, Savage, Goggs, Fischer von Mollard, Poole: "Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability." in: The Journal of biological chemistry, Vol. 290, Issue 3, pp. 1536-45, (2015) (PubMed).

Target Details for VAMP8

Target: VAMP8 (Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8))

Alternative Name: Endobrevin (VAMP8 Products)

Synonyms: vamp8 antibody, EDB antibody, VAMP-8 antibody, AU041171 antibody, Edb antibody, endobrevin antibody, wu:fa18d07 antibody, zgc:56588 antibody, vesicle associated membrane protein 8 antibody, vesicle-associated membrane protein 8 (endobrevin) antibody, vesicle-associated membrane protein 8 antibody, vamp8 antibody, VAMP8 antibody, Vamp8 antibody

Background: Synonyms: VAMP 8