SYT1 antibody (Lumenal Region)

Catalog No. ABIN1742201

Product Details anti-SYT1 Antibody

Target: SYT1 (Synaptotagmin I (SYT1))

Binding Specificity: AA 1-8, Lumenal Region

Reactivity: Rat, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Specific for synaptotagmin 1

Cross-Reactivity (Details): no cross-reactivity to synaptotagmin 2.

Purification: Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization.

Immunogen: Synthetic peptide MVSASRPE (aa 1-8 in mouse synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 2000 (AP staining)
IP: not tested yet
IHC: not tested yet

Comment:

This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-SYT1 antibody (ABIN1742201)

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, (2014) (PubMed).

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin 1 (SYT1 Products)

Synonyms: P65 antibody, SVP65 antibody, SYT antibody, CG3139 antibody, D. Syt I antibody, DSYT antibody, DSYT2 antibody, DSypt antibody, DSyt antibody, Dmel\\CG3139 antibody, Droso1 antibody, Dsyt2 antibody, SYT I antibody, SYT1 antibody, Syt antibody, Syt 1 antibody, Syt I antibody, Syt-1 antibody, SytI antibody, dSYT antibody, dsyt1 antibody, dsytI antibody, l(2)23AB1 antibody, l(2)23Ba antibody, l(2)k05909 antibody, syt antibody, syt 1 antibody, syt I antibody, syt-1 antibody, syt1 antibody, sytI antibody, AW124717 antibody, G630098F17Rik antibody, Syti antibody, GB20036 antibody, DKFZP459P193 antibody, svp65 antibody, p65 antibody, wu:fj41c01 antibody, wu:fk56d05 antibody, zgc:92899 antibody, synaptotagmin 1 antibody, Synaptotagmin 1 antibody, synaptotagmin I antibody, synaptotagmin-1 antibody, synaptotagmin, p65 antibody, synaptotagmin Ia antibody, synaptotagmin 1 L homeolog antibody, SYT1 antibody, Syt1 antibody, Tsp_06980 antibody, Tsp_08220 antibody, syt1 antibody, LOC100533340 antibody, syt1a antibody, syt1.L antibody

Background: Synonyms: p65

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport