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GLRB antibody (AA 336-355, AA 336-455)

GLRB Reactivity: Mouse, Rat, Monkey ICC, IHC Host: Mouse Monoclonal 299E7 unconjugated
Catalog No. ABIN1742318
  • Target See all GLRB Antibodies
    GLRB (Glycine Receptor, beta (GLRB))
    Binding Specificity
    • 15
    • 10
    • 9
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 336-355, AA 336-455
    Reactivity
    • 35
    • 32
    • 21
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse, Rat, Monkey
    Host
    • 42
    • 1
    Mouse
    Clonality
    • 42
    • 1
    Monoclonal
    Conjugate
    • 16
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GLRB antibody is un-conjugated
    Application
    • 39
    • 23
    • 13
    • 13
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Specificity
    Specific for the glycine receptor beta-subunit.
    Purification
    purified IgG
    Immunogen
    Recombinant protein (aa 336-455 of rat GlyR beta) Epitope aa 336-355
    Clone
    299E7
    Isotype
    IgG1
    Top Product
    Discover our top product GLRB Primary Antibody
  • Application Notes
    WB: not recommended
    IP: not tested yet
    IHC: 1 : 500 up to 1 : 1000
    Comment

    WB: Detection of overexpressed protein is possible. Endogenous levels are hardly detectable. IHC: Mild fixation for 10-15 min with cold 2 % PFA solution is recommended. Include 0,5 % triton X 100 in all blocking and antibody incubation steps.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS, 0.02% sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Storage
    -20 °C
    Storage Comment
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Weltzien, Puller, OSullivan, Paarmann, Betz: "Distribution of the glycine receptor β-subunit in the mouse CNS as revealed by a novel monoclonal antibody." in: The Journal of comparative neurology, Vol. 520, Issue 17, pp. 3962-81, (2012) (PubMed).

  • Target
    GLRB (Glycine Receptor, beta (GLRB))
    Alternative Name
    Glycine Receptor beta (GLRB Products)
    Synonyms
    HKPX2 antibody, glrb antibody, MGC145156 antibody, AI853901 antibody, Glyrb antibody, spa antibody, spastic antibody, glrb2 antibody, zgc:101041 antibody, glycine receptor beta antibody, glycine receptor, beta antibody, glycine receptor, beta subunit antibody, glycine receptor, beta b antibody, GLRB antibody, Glrb antibody, glrb antibody, glrbb antibody
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