SNAPIN antibody (C-Term)

Catalog No. ABIN1742322

Product Details anti-SNAPIN Antibody

Target: SNAPIN (SNAP-Associated Protein (SNAPIN))

Binding Specificity: AA 117-136, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAPIN antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for snapin.

Purification: antiserum

Immunogen: Synthetic peptide TARRRAMLDSGVYPPGSPSK (aa 117-136 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 100 - 1 : 5000 (AP staining)
IP: not recommended
ICC: not tested yet
IHC: not recommended

Comment:

Since snapin is present in very low concentrations long exposure time is recommended.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SNAPIN antibody (ABIN1742322)

Zhou, Cai, Xie, Sheng: "Snapin recruits dynein to BDNF-TrkB signaling endosomes for retrograde axonal transport and is essential for dendrite growth of cortical neurons." in: Cell reports, Vol. 2, Issue 1, pp. 42-51, (2012) (PubMed).

Zhang, Wada, Yasuhara, Iseda, Eguchi, Fukui, Yang, Yamagata, Hiesberger, Igarashi, Zhang, Wang, Akagi, Kanwar, Makino: "The role for HNF-1beta-targeted collectrin in maintenance of primary cilia and cell polarity in collecting duct cells." in: PLoS ONE, Vol. 2, Issue 5, pp. e414, (2007) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Vites, Rhee, Schwarz, Rosenmund, Jahn: "Reinvestigation of the role of snapin in neurotransmitter release." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26251-6, (2004) (PubMed).

Target Details for SNAPIN

Target: SNAPIN (SNAP-Associated Protein (SNAPIN))

Alternative Name: Snapin (SNAPIN Products)

Background: Synonyms: Snapap

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis