CACNA1B antibody (AA 1921-2222)

Catalog No. ABIN1742328

Product Details anti-CACNA1B Antibody

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Binding Specificity: AA 1921-2222

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for Ca2+ channel alpha-1E.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein (aa 1921-2222 of rat Ca2+-channel alpha-1E subunit).

Application Details

Application Notes: WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500

Comment:

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for CACNA1B

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Alternative Name: Ca2+ Channel (CACNA1B Products)

Synonyms: BIII antibody, CACNL1A5 antibody, CACNN antibody, Cav2.2 antibody, AW050276 antibody, AW060892 antibody, AW822256 antibody, Cchn1a antibody, alpha(1B) antibody, calcium voltage-gated channel subunit alpha1 B antibody, calcium channel, voltage-dependent, N type, alpha 1B subunit antibody, CACNA1B antibody, Cacna1b antibody

Background: Synonyms: Cav2.3