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VAMP5 antibody (AA 1-70)

VAMP5 Reactivity: Mouse WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1742369
  • Target See all VAMP5 Antibodies
    VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))
    Binding Specificity
    • 5
    • 3
    • 2
    • 1
    • 1
    AA 1-70
    Reactivity
    • 18
    • 5
    • 3
    Mouse
    Host
    • 16
    • 4
    • 1
    Rabbit
    Clonality
    • 19
    • 3
    Polyclonal
    Conjugate
    • 12
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This VAMP5 antibody is un-conjugated
    Application
    • 9
    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Specificity
    Specific for myobrevin.
    Purification
    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
    Immunogen
    Recombinant mouse myobrevin (aa 1-70).
  • Application Notes
    WB: 1 : 1000 up to 1 : 5000 (AP staining)
    IP: not tested yet
    ICC: 1 : 1000
    IHC-P: 1 : 200
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handling Advice
    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
    Storage
    -20 °C
    Storage Comment
    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Ikezawa, Tajika, Ueno, Murakami, Inoue, Yorifuji: "Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung." in: Developmental dynamics : an official publication of the American Association of Anatomists, (2017) (PubMed).

    Sadler, Bryant, Gould: "Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking." in: Molecular biology of the cell, Vol. 26, Issue 3, pp. 530-6, (2015) (PubMed).

    Takahashi, Tajika, Khairani, Ueno, Murakami, Yorifuji: "The localization of VAMP5 in skeletal and cardiac muscle." in: Histochemistry and cell biology, Vol. 139, Issue 4, pp. 573-82, (2013) (PubMed).

    Rose, Jeppesen, Kiens, Richter: "Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 297, Issue 5, pp. R1228-37, (2009) (PubMed).

  • Target
    VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))
    Alternative Name
    Myobrevin (VAMP5 Products)
    Synonyms
    AF119384 antibody, Camp antibody, vesicle associated membrane protein 5 antibody, vesicle-associated membrane protein 5 antibody, VAMP5 antibody, vamp5 antibody, Vamp5 antibody
    Background
    Synonyms: VAMP 5
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