GRIK5 antibody (C-Term)

Catalog No. ABIN1742375

Product Details anti-GRIK5 Antibody

Target: GRIK5 (Glutamate Receptor, Ionotropic, Kainate 5 (GRIK5))

Binding Specificity: AA 828-979, C-Term

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for GluK 5.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein of rat GluK 5 (C-terminus, aa 828-979).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000

Comment:

IHC: Antibody requires Clarke liquid fixation (1 vol. acetic acid, 6 vol. ethanol, 15 min at RT).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-GRIK5 antibody (ABIN1742375)

Fujikawa, Petralia, Fitzgerald, Wang, Millis, Morgado-Díaz, Kitamura, Kachar: "Localization of kainate receptors in inner and outer hair cell synapses." in: Hearing research, Vol. 314, pp. 20-32, (2014) (PubMed).

Marrocco, Mairesse, Ngomba, Silletti, Van Camp, Bouwalerh, Summa, Pittaluga, Nicoletti, Maccari, Morley-Fletcher: "Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 48, pp. 17143-54, (2012) (PubMed).

Target Details for GRIK5

Target: GRIK5 (Glutamate Receptor, Ionotropic, Kainate 5 (GRIK5))

Alternative Name: KA 2 (GRIK5 Products)

Synonyms: EAA2 antibody, GRIK2 antibody, GluK5 antibody, KA2 antibody, GRIK5 antibody, Glur6 antibody, ka2 antibody, eaa2 antibody, GluRgamma2 antibody, iGlu5 antibody, glutamate ionotropic receptor kainate type subunit 5 antibody, glutamate receptor, ionotropic, kainate 5 antibody, glutamate ionotropic receptor kainate type subunit 2 antibody, glutamate receptor, ionotropic, kainate 5 L homeolog antibody, glutamate receptor, ionotropic, kainate 5 (gamma 2) antibody, GRIK5 antibody, GRIK2 antibody, grik5 antibody, grik5.L antibody, Grik5 antibody

Background: Synonyms: GluR-gamma2

Pathways: Carbohydrate Homeostasis, Synaptic Membrane, Maintenance of Protein Location, Synaptic Vesicle Exocytosis