Ribeye (AA 974-988) antibody

Catalog No. ABIN1742396

Product Details

Target: Ribeye

Binding Specificity: AA 974-988

Reactivity: Mouse, Rat, Cow, Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: This antibody recognizes ribeye and CtBP 2 (cat. no. 193 003).

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide corresponding to aa 974-988 of rat ribeye coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 10000

Comment:

The immunogen is identical to that of anti-CtBP 2 (ABIN1742398).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Zabouri, Haverkamp: "Calcium channel-dependent molecular maturation of photoreceptor synapses." in: PLoS ONE, Vol. 8, Issue 5, pp. e63853, (2013) (PubMed).

Müller, Butz, Peichl, Haverkamp: "The rod pathway of the microbat retina has bistratified rod bipolar cells and tristratified AII amacrine cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1014-23, (2013) (PubMed).

Hübler, Rankovic, Richter, Lazarevic, Altrock, Fischer, Gundelfinger, Fejtova: "Differential spatial expression and subcellular localization of CtBP family members in rodent brain." in: PLoS ONE, Vol. 7, Issue 6, pp. e39710, (2012) (PubMed).

Target Details

Target: Ribeye