Ribeye (AA 95-207) antibody

Catalog No. ABIN1742397

Product Details

Target: Ribeye

Binding Specificity: AA 95-207

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: This antibody recognizes only ribeye and not CtBP 2.

Cross-Reactivity (Details): Other species not tested yet.

Characteristics: A scaffolding protein of the ribbon synapse

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein corresponding to AA 95 to 207 from rat Ribeye (UniProt Id: Q9EQH5-2)

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Fairchild, Hino, Han, Miltner, Peinado Allina, Brown, Burns, La Torre, Simó: "RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion." in: Development (Cambridge, England), Vol. 145, Issue 3, (2018) (PubMed).

Carrott, Bowl, Aguilar, Johnson, Chessum, West, Morse, Dorning, Smart, Hardisty-Hughes, Ball, Parker, Barnard, MacLaren, Wells, Marcotti, Brown: "Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 1, pp. 222-34, (2016) (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Fujikawa, Petralia, Fitzgerald, Wang, Millis, Morgado-Díaz, Kitamura, Kachar: "Localization of kainate receptors in inner and outer hair cell synapses." in: Hearing research, Vol. 314, pp. 20-32, (2014) (PubMed).

Regus-Leidig, Fuchs, Löhner, Leist, Leal-Ortiz, Chiodo, Hauswirth, Garner, Brandstätter: "In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses." in: Frontiers in cellular neuroscience, Vol. 8, pp. 259, (2014) (PubMed).

Tekin, Chioza, Matsumoto, Diaz-Horta, Cross, Duman, Kokotas, Moore-Barton, Sakoori, Ota, Odaka, Foster, Cengiz, Tokgoz-Yilmaz, Tekeli, Grigoriadou, Petersen, Sreekantan-Nair, Gurtz, Xia, Pandya et al.: "SLITRK6 mutations cause myopia and deafness in humans and mice. ..." in: The Journal of clinical investigation, Vol. 123, Issue 5, pp. 2094-102, (2013) (PubMed).

Fuchs, Scholz, Sendelbeck, Atorf, Schlegel, Enz, Brandstätter: "Rod photoreceptor ribbon synapses in DBA/2J mice show progressive age-related structural changes." in: PLoS ONE, Vol. 7, Issue 9, pp. e44645, (2012) (PubMed).

Hübler, Rankovic, Richter, Lazarevic, Altrock, Fischer, Gundelfinger, Fejtova: "Differential spatial expression and subcellular localization of CtBP family members in rodent brain." in: PLoS ONE, Vol. 7, Issue 6, pp. e39710, (2012) (PubMed).

Regus-Leidig, tom Dieck, Brandstätter: "Absence of functional active zone protein Bassoon affects assembly and transport of ribbon precursors during early steps of photoreceptor synaptogenesis." in: European journal of cell biology, Vol. 89, Issue 6, pp. 468-75, (2010) (PubMed).

Target Details

Target: Ribeye