GAD65 antibody (AA 3-96)

Catalog No. ABIN1742417

Product Details anti-GAD65 Antibody

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Binding Specificity: AA 3-96

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for GAD 2 / GAD 65

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Purified recombinant mouse GAD 2 (aa 3-96).

Clone: 26H1

Isotype: IgG3

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200 up to 1 : 500

Comment:

WB: This antibody is less sensitive than the rabbit antibody. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742419 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-GAD65 antibody (ABIN1742417)

Pan, Marrs, Ryan: "Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).

Liu, Bickford, Held, Nyitrai, Südhof, Kaeser: "The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 37, pp. 12289-303, (2014) (PubMed).

Coley, Ruffin, Moss, Hopfer, Boron: "Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons." in: Neuroscience, Vol. 246, pp. 451-67, (2013) (PubMed).

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD 2 (GAD2 Products)

Synonyms: GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody

Background: Synonyms: GAD 65