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alpha Tubulin antibody (AA 443-449)

TUBA1 Reactivity: Human, Mouse, Rat, Chicken, Mammalian WB, ELISA, IHC, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1742550
  • Target See all alpha Tubulin (TUBA1) Antibodies
    alpha Tubulin (TUBA1)
    Binding Specificity
    • 16
    • 11
    • 6
    • 6
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 443-449
    Reactivity
    • 108
    • 73
    • 66
    • 24
    • 19
    • 15
    • 14
    • 13
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Mammalian
    Host
    • 61
    • 55
    • 8
    • 2
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 72
    • 55
    • 2
    Polyclonal
    Conjugate
    • 84
    • 11
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Tubulin antibody is un-conjugated
    Application
    • 102
    • 44
    • 42
    • 42
    • 37
    • 33
    • 27
    • 14
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Specificity
    Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
    Purification
    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
    Immunogen
    Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
    Top Product
    Discover our top product TUBA1 Primary Antibody
  • Application Notes
    WB: 1 : 1000 up to 1 : 5000 (AP staining)
    IP: not tested yet
    ICC: 1 : 100 up to 1 : 1000
    IHC: 1 : 400
    Comment

    ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handling Advice
    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
    Storage
    -20 °C
    Storage Comment
    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

  • Target
    alpha Tubulin (TUBA1)
    Alternative Name
    alpha-Tubulin (TUBA1 Products)
    Synonyms
    Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody
    Pathways
    Microtubule Dynamics
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