alpha Tubulin antibody (AA 443-449)
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- Target See all alpha Tubulin (TUBA1) Antibodies
- alpha Tubulin (TUBA1)
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Binding Specificity
- AA 443-449
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Reactivity
- Human, Mouse, Rat, Chicken, Mammalian
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This alpha Tubulin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
- Top Product
- Discover our top product TUBA1 Primary Antibody
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- Application Notes
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WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC: 1 : 400 - Comment
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ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742547 or ABIN1742549 as capture antibodies (protocol for sandwich-ELISA).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "
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Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
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- Target
- alpha Tubulin (TUBA1)
- Alternative Name
- alpha-Tubulin (TUBA1 Products)
- Synonyms
- Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody
- Pathways
- Microtubule Dynamics
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