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MICA antibody

MICA Reactivity: Human, Cow, Cat, Non-Human Primate ELISA, FACS, IP, ICC, IHC (fro), Func, CyTOF Host: Mouse Monoclonal W6-32 unconjugated
Catalog No. ABIN1774789
  • Target See all MICA Antibodies
    MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
    Reactivity
    • 69
    • 17
    • 13
    • 11
    • 6
    • 5
    • 2
    • 1
    Human, Cow, Cat, Non-Human Primate
    Host
    • 37
    • 32
    Mouse
    Clonality
    • 37
    • 33
    Monoclonal
    Conjugate
    • 44
    • 8
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MICA antibody is un-conjugated
    Application
    • 45
    • 36
    • 32
    • 18
    • 17
    • 11
    • 9
    • 7
    • 6
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Cytometry by Time of Flight (CyTOF)
    Specificity
    The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
    No Cross-Reactivity
    Rabbit
    Cross-Reactivity (Details)
    Human, Non-Human Primates, Bovine, Feline (Cat)
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Endotoxin Level
    Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
    Immunogen
    Membrane of human tonsil cells
    Clone
    W6-32
    Isotype
    IgG2a
    Top Product
    Discover our top product MICA Primary Antibody
  • Application Notes
    Functional application: The antibody W6/32 is suitable as a positive control for HLA tissue typing.
    Flow cytometry: Recommended dilution: 1-4 μg/mL
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4
    Preservative
    Azide free
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." in: Human immunology, Vol. 64, Issue 11, pp. 1039-44, (2003) (PubMed).

    Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." in: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, (2001) (PubMed).

    Ladasky, Shum, Canavez, Seuánez, Parham: "Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody." in: Immunogenetics, Vol. 49, Issue 4, pp. 312-20, (1999) (PubMed).

    Shields, Ribaudo: "Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin." in: Tissue antigens, Vol. 51, Issue 5, pp. 567-70, (1998) (PubMed).

    Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, (1994) (PubMed).

    Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, (1987) (PubMed).

    Neefjes, Hensen, de Kroon, Ploegh: "A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes." in: Immunogenetics, Vol. 23, Issue 5, pp. 341-7, (1986) (PubMed).

    Brodsky, Parham: "Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies." in: Immunogenetics, Vol. 15, Issue 2, pp. 151-66, (1982) (PubMed).

    Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." in: Cell, Vol. 14, Issue 1, pp. 9-20, (1978) (PubMed).

  • Target
    MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
    Alternative Name
    HLA-Class I (MICA Products)
    Synonyms
    MIC-A antibody, PERB11.1 antibody, MHC class I polypeptide-related sequence A antibody, MICA antibody
    Background
    HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
    Pathways
    Activation of Innate immune Response, Transition Metal Ion Homeostasis
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