CCR4 antibody

Antigen: CCR4

Clonality: Monoclonal (KH-4F5)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA), Immunofluorescence (IF)

Catalog no.: ABIN179723

Additional Information

Immunogen: immunisation with human CCR4 peptide

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: KH-4F5

Description: CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupled receptors. Chemokine receptors are membrane-bound molecules composed of 7-transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds the chemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positive CD4(+) thymocytes especially in TH2- and regulatory T-cells (3,4). It plays a central role in T cell migration to the thymus, T cell maturation and education. CCR4 is often up-regulated in inflammation and cause conformational changes that trigger intracellular signaling pathways (2,5).

Specificity: Anti-human CCR4

Application Details

Application Notes: FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400, IF: 1ug / 10^6 cells

Concentration: 2 mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)

Research Area: Chemokines, Receptors

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using KH-4F5. BOSC23 cells were transientlytransfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of KH-4F5 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.

Spectral Confocal Microscopy of CHO cells using KH-4F5. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with A

SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified KH-4F5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain Reagent.

Publications

Publications: Imai, Baba, Nishimura et al.: "The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 15036-42, 1997 (PubMed).

Imai, Chantry, Raport et al.: "Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1764-8, 1998 (PubMed).

Murdoch, Finn: "Chemokine receptors and their role in inflammation and infectious diseases." in: Blood, Vol. 95, Issue 10, pp. 3032-43, 2000 (PubMed).

Andrew, Ruffing, Kim et al.: "C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 103-11, 2001 (PubMed).

Purandare, Somerville: "Antagonists of CCR4 as immunomodulatory agents." in: Current topics in medicinal chemistry, Vol. 6, Issue 13, pp. 1335-44, 2006 (PubMed).