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Antibodies
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anti-Phosphotyrosine (0) antibody (Alexa Fluor 488)
Phosphotyrosine (0) antibody (Alexa Fluor 488)
| Antigen |
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| Clonality |
Monoclonal (PY20) |
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Host
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Reactivity
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Conjugate
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Application
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Western Blotting (WB) (140), Immunoprecipitation (IP) (88), ELISA (61), Enzyme Immunoassay (EIA) (37), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunocytochemistry (ICC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), To be determined by user (TBD) (2), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN179732 |
| Quantity |
100 µg |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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Phosphotyrosine
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Immunogen
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KLH-conjugated phosphotyrosine
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Isotype
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IgG2b, kappa chain
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Clone
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PY20
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Description
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Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining. Distribution: Phospho-Specific Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
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Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunofluorescence microscopy. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Purification
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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Storage
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The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Research Area
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Amino Acids, Protein Modifications
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Restrictions
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For Research Use only
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Publications
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Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).
Vuori, Ruoslahti: "Tyrosine phosphorylation of p130Cas and cortactin accompanies integrin-mediated cell adhesion to extracellular matrix." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22259-62, 1995 (PubMed).
Prahalad, Calvo, Waechter et al.: "Regulation of MDCK cell-substratum adhesion by RhoA and myosin light chain kinase after ATP depletion." in: American journal of physiology. Cell physiology, Vol. 286, Issue 3, pp. C693-707, 2004 (PubMed).
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Alternatives for antigen "Phosphotyrosine (0)", type "Antibodies"
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Hosts
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Mouse (138), Rabbit (31), Armenian Hamster (2), Goat (2)
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Reactivities
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Human (22), Rat (Rattus) (18), Dog (Canine) (13), Mouse (Murine) (13), Various Species (4), All Species (2), Chicken (1), Cow (Bovine) (1)
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Applications
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Western Blotting (WB) (140), Immunoprecipitation (IP) (88), ELISA (61), Enzyme Immunoassay (EIA) (37), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunocytochemistry (ICC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), To be determined by user (TBD) (2), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (26), HRP (18), FITC (14), PE (7), Agarose Beads (5), Alkaline Phosphatase (AP) (5), RPE (2), TAMRA (2), Rhodamine (1)
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Alternatives 
