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BrdU antibody
| Antigen | BrdU |
| Clonality | Monoclonal (MoBU-1) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives All Species |
| Application |
Alternatives Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (IHC)
|
 |
3 references available |
| Catalog no. | ABIN179736 |
| Quantity | 100 µg (Variants) |
| Price | Product not available in this region. |
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Additional Information
| Format | Purified |
| Isotype | IgG1, kappa chain (Matching secondary antibodies) |
| Clone | MoBU-1 |
| Description | BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval. The MoBU-1 antibody is useful for the detection of incorporated BrdU by fluorescent cell staining (ICFC), flow cytometry, and immunohistochemistry. Distribution: Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle. Function: Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation. Synonyms: Bromodeoxyuridine Structure: Uridine derivative that can be incorporated into DNA and substitute for thymidine residues. |
Application Details
| Application Notes | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C. |
| Restrictions | For Research Use only |
Publications
| Publications |
Dolbeare, Beisker, Pallavicini et al.: "Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity." in: Cytometry, Vol. 6, Issue 6, pp. 521-30, 1986 (PubMed).
Dolbeare, Gratzner, Pallavicini et al.: "Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 18, pp. 5573-7, 1983 (PubMed). Gratzner: "Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication." in: Science (New York, N.Y.), Vol. 218, Issue 4571, pp. 474-5, 1982 (PubMed). |
Alternatives
Alternatives for antigen "BrdU", type "Antibodies"
| Hosts | Rabbit (41), Mouse (14), Rat (6), Sheep (2) |
| Reactivities | Compound (28), Human (13), Mouse (Murine) (12), Rat (Rattus) (12), Dog (Canine) (5), Various Species (5), Rabbit (4), Sheep (Ovine) (4), Chicken (3), Cow (Bovine) (3), Pig (Porcine) (3), Horse (Equine) (1) |
| Applications | Flow Cytometry (FACS) (51), Immunofluorescence (IF) (41), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunohistochemistry (IHC) (7), ELISA (6), Western Blotting (WB) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1) |
| Conjugates | FITC (7), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Alexa Flour 488 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2) |
