Neurofilament Heavy Protein antibody

Antigen: Neurofilament Heavy Protein

Clonality: Monoclonal (NF-01)

Host: Mouse

Reactivity: All Species

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN179740

Additional Information

Alternative name: Neurofilament heavy protein (NF-H)

Immunogen: Pellet of pig brain cold stable proteins after microtubule depolymerization

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: NF-01

Description: NF-H is an abundant, stable cytoplasmic protein located in neuronal cells in large axons frequently used as a cell type marker. The NF-H protein shares a high degree of structural and sequence homology with the NF-L and NF-M subunits, especially in the coiled-coil core domain. NF-M and NF-H form flexible extensions linking the neurofilament proteins to each other and other cytoplasmic proteins. Deletions and insertions in the NF-H protein have been reported in amyotrophic lateral sclerosis. The NF-01 monoclonal antibody reacts with a conserved phosphorylated epitope of NF-H present in all species and has been reported to be useful for Western blotting, immunohistochemistry using formalin-fixed, paraffin-embedded tissues and immunofluorescence staining. Distribution: Cytoplasmic protein, neuronal cells (large axons) Function: Provides flexible extension (along with NF-M) to link neurofilaments to each other and to other cytoplasmic proteins Modifications: Phosphorylation Synonyms: Neurofilament protein, heavy polypeptide, neurofilament heavy polypeptide Structure: Contains four coiled-coil core domains, in most vertebrates NF-H forms interacts with NF-M (medium chains) and NF-L (light chains), approximately 210 kD. NF-H contains a repetitive sequence motif (Lys-Ser-Pro) repeated more than 40 times.

Specificity: Reacts with conserved phosphorylated epitope Syntaxin binding protein 1, Neurofilament protein medium polypeptide, Neurofilament protein light polypeptide. reacts with conserved phosphorylated epitope

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections, immunocytochemistry. Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.

Storage: Upon receipt, store undiluted at 4° C.

Restrictions: For Research Use only

Images

Lysates from primary human brain tissues were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against NF-H. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Publications

Publications: Lukuas, Druaber, Bucek et al.: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, 1994 (PubMed).