Tubulin, alpha (TUBA) antibody (Biotin)

Antigen: Tubulin, alpha (TUBA)

Synonyms: Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)Tom, Fs(3)Tomaj, Kavar, T, Tom, Tub, aTub67C, alpha-Tub, alpha-Tub67C, alpha-tubulin, alpha-tubulin[4], alpha4, alpha4-t, alpha4-tubulin, alpha4Tub67C, alpha4t, alpha67C, alphaTUB, alphaTub4, alphaTub[67C], alphatub67C, mum-tub67C, DmelCG8308, CG8308, 1t, ALPHA 84C, BEST:LD32507, DM1alpha, DTA1, Talpha1, Tuba84B, Tubalpha1, a1t, aTub84B, agr;Tub, alpha-Tub84B, alpha1, alpha1t, alpha1tub, alpha84B, alphaT, alphaTub1, alphat-1, anon-EST:Liang-1.59, anon-EST:Liang-2.30, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tubulin, DmelCG1913, CG1913, 3t, ALPHA 84D, D.m.ALPHA-84D, DTA4, aTub84D, alpha-Tub84D, alpha3, alpha3t, alpha84D, alphaTub3, DmelCG2512, CG2512, 2t, ALPHA 85E, DTA3, a2t, aTub85E, alpha-Tub85E, alpha2, alpha2t, alpha85E, alpha85E-tub, alphaTub2, alphaTub84E, DmelCG9476, CG9476, TUBA3E, TUBA2, LIS3, TUBA3, FLJ25113, B-ALPHA-1, Tuba3b, Tuba3a, RGD1565155, TUBA3D, MGC133894, tua3

Clonality: Monoclonal (10D8)

Host: Mouse

Reactivity: All Species, Human, Mouse (Murine), Rat (Rattus)

Conjugate: Biotin

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN179742

Additional Information

Alternative name: Tubulin-alpha

Immunogen: Recombinant full length human alpha-tubulin

Isotype: IgM, kappa chain

Clone: 10D8

Description: Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes alpha-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting. Distribution: Ubiquitous Function: Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site Modifications: Phosphorylation, acetylation Synonyms: alpha-tubulin, Tubulin, alpha-ubiquitous chain Structure: Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.

Specificity: Recognized epitope conserved across species RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A.

Synonyms: Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)Tom, Fs(3)Tomaj, Kavar, T, Tom, Tub, aTub67C, alpha-Tub, alpha-Tub67C, alpha-tubulin, alpha-tubulin[4], alpha4, alpha4-t, alpha4-tubulin, alpha4Tub67C, alpha4t, alpha67C, alphaTUB, alphaTub4, alphaTub[67C], alphatub67C, mum-tub67C, DmelCG8308, CG8308, 1t, ALPHA 84C, BEST:LD32507, DM1alpha, DTA1, Talpha1, Tuba84B, Tubalpha1, a1t, aTub84B, agr;Tub, alpha-Tub84B, alpha1, alpha1t, alpha1tub, alpha84B, alphaT, alphaTub1, alphat-1, anon-EST:Liang-1.59, anon-EST:Liang-2.30, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tubulin, DmelCG1913, CG1913, 3t, ALPHA 84D, D.m.ALPHA-84D, DTA4, aTub84D, alpha-Tub84D, alpha3, alpha3t, alpha84D, alphaTub3, DmelCG2512, CG2512, 2t, ALPHA 85E, DTA3, a2t, aTub85E, alpha-Tub85E, alpha2, alpha2t, alpha85E, alpha85E-tub, alphaTub2, alphaTub84E, DmelCG9476, CG9476, TUBA3E, TUBA2, LIS3, TUBA3, FLJ25113, B-ALPHA-1, Tuba3b, Tuba3a, RGD1565155, TUBA3D, MGC133894, tua3

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Research Area: Cytoskeleton, Cell/Tissue Markers

Restrictions: For Research Use only

Images

Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-a-tubulin (Clone 10D8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Publications

Publications: Chou, Chou, Lee: "CDK-dependent activation of poly(ADP-ribose) polymerase member 10 (PARP10)." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15201-7, 2006 (PubMed).