Keratin (KRT) (pan reactive) antibody

Antigen: Keratin (KRT)

Synonyms: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Epitope: pan reactive

Clonality: Monoclonal (C-11)

Host: Mouse

Reactivity: All Species

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN179743

Additional Information

Alternative name: Cytokeratin (pan reactive)

Immunogen: Keratin enriched fraction from human epidermoid carcinoma

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: C-11

Description: Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus. The C-11 monoclonal antibody reacts with conserved epitope in all species tested of cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry. Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal ant-cytokeratin antibody (clone C-11). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Distribution: Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus Function: Intermediate filament protein involved with the cytoskeleton. Synonyms: Cytokeratin, Keratin Structure: Itermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits

Specificity: Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others. reacts with conserved epitope in all species tested. recognizes cytokeratin 4

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.

Storage: The antibody solution should be stored undiluted at 4 °C.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Images

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal ant-cytokeratin antibody (clone C-11). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Publications

Publications: Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Kovaruik, Rejthar, Lauerovua et al.: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989 (PubMed).

Chernyavsky, Arredondo, Kitajima et al.: "Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens." in: The Journal of biological chemistry, Vol. 282, Issue 18, pp. 13804-12, 2007 (PubMed).