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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

Antigen

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Synonyms
G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, G ... show more
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus), Rabbit
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN179746
Quantity 125 µl  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name GAPDH
Immunogen Peptide-KLH (C-terminal)
Format Purified
Isotype IgG  (Matching secondary antibodies)
Clone Poly6314
Description This rabbit polyclonal antibody recognizes human, mouse, rat, and rabbit GAPDH also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 37 kDa. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. The poly6314 antibody has been shown to be useful for Western blotting. Distribution: Ubiquitously expressed Function: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme is thought to be a tetramer of identical chains. Synonyms: Glyceraldehyde 3 phosphate dehydrogenase Structure: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 37 kD.
Specificity Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2 proteins.
Synonyms G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 25 µl antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Purification The antibody was purified by antigen-affinity chromatography.
Buffer This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.1% gelatin.
Storage Upon receipt, store at 4° C
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts Rabbit (141), Mouse (124), Goat (23), Sheep (13), Chicken (2)
Reactivities Human (205), Mouse (Murine) (122), Rat (Rattus) (115), Pig (Porcine) (37), Rabbit (36), Cow (Bovine) (31), Dog (Canine) (21), Chicken (17), Goat (13), Fish (12), Hamster (9), Escherichia Coli (E. Coli) (8), Cat (Feline) (7), Monkey (7), Mammalian (6), Bird (Avian) (5), Yeast (Saccharomyces Cerevisiae) (5), Fruit Fly (Drosophila melanogaster) (3), Insect (3), Xenopus laevis (3), Sheep (Ovine) (2), Amphibian (1), Bacteria (1), Horse (Equine) (1), Saccharomyces cerevisiae (S. cerevisiae) (1), Zebrafish (Brachydanio rerio) (1)
Applications Western Blotting (WB) (220), Immunofluorescence (IF) (147), ELISA (89), Flow Cytometry (FACS) (80), Enzyme Immunoassay (EIA) (45), Immunoprecipitation (IP) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Immunocytochemistry (ICC) (21), Immunohistochemistry (IHC) (18), Dot Blot (Dot) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Immunodiffusion (ID) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Radioimmunoassay (RIA) (6), Immunoelectron Microscopy (IEM) (3), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (1)
Conjugates HRP (15), Biotin (13), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Alexa Flour 488 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy7 (4), RBITC (4)
Epitopes C-Term (13), N-Term (6), internal (3)