Neurofilament Medium Protein antibody

Antigen: Neurofilament Medium Protein

Clonality: Monoclonal (NF-09)

Host: Mouse

Reactivity: All Species

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN179764

Additional Information

Alternative name: Neurofilament medium protein (NF-M)

Immunogen: Pellet of pig brain cold stable proteins after microtubule depolymerization

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: NF-09

Description: NF-M is an abundant, stable cytoplasmic protein located in neuronal cells in large axons frequently used as a cell type specific marker. NF-M is modified by glycosylation and phosphorylation. The NF-M protein shares a high degree of structural and sequence homology with the NF-L and NF-H subunits, especially in the coiled-coil core domain. NF-M and NF-H form flexible extensions linking the neurofilament proteins to each other and other cytoplasmic proteins. The NF-09 monoclonal antibody recognizes the highly conserved NF-M protein (phosphorylation and non-phosphorylated form) in all species.The NF-09 antibody has been reported to be useful for Western blotting, immunohistochemistry using formalin-fixed paraffin-embedded tissues, and immunofluorescence staining. Distribution: Cytoplasmic protein, neuronal cells (large axons) Function: Provides flexible extension (along with NF-H) to link neurofilaments to each other and to other cytoplasmic proteins Modifications: Glycosylation, Phosphorylation (S511, S545, S615, S620, S736, S837) Synonyms: Neurofilament protein, medium polypeptide, neurofilament medium polypeptide Structure: Contains four coiled-coil core domains, in most vertebrates NF-M forms interacts with NF-H (heavy chains) and NF-L (light chains), approximately 160 kD.

Specificity: Reacts with both phosphorylated/non-phosphorylated forms Syntaxin binding protein 1, Neurofilament protein heavy polypeptide, Neurofilament protein light polypeptide, Alipoprotein E. reacts with both phosphorylated and non-phosphorylated forms

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry (FFPE sections), immunocytochemistry. Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.

Storage: Upon receipt, store undiluted at 4° C.

Restrictions: For Research Use only

Images

Lysates from primary human brain tissues were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against NF-M. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Publications

Publications: Druaberovua, Sulimenko, Kukharskyy et al.: "Monoclonal antibody NF-09 specific for neurofilament protein NF-M." in: Folia biologica, Vol. 45, Issue 4, pp. 163-5, 2000 (PubMed).