MCP-1 (CCL2) antibody (Biotin)

Antigen: MCP-1 (CCL2)

Clonality: Monoclonal (2H5)

Host: Hamster

Reactivity: Mouse (Murine), Rat (Rattus), Human

Conjugate: Biotin

Application: ELISA (Detection), ELISPOT (Detection), Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN179780

Additional Information

Alternative name: MCP-1

Immunogen: CHO-expressed, recombinant mouse MCP-1

Isotype: IgG

Clone: 2H5

Description: Chemoattractant, regulates adhesion molecule expression, cytokine production in monocytes, proliferation/activation of CC-chemokine activated killer cells Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1. Synonyms: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2 Regulation: Upregulated by IL-1, TNF-alpha, PDGF, TGF-beta Structure: CC chemokine, dimer, 13.8 kD (Mammalian)

Application Details

Application Notes: ELISA or ELISPOT Capture: The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (ABIN160442) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (ABIN160249) as the detecting antibody for the detection of human MCP-1. The LEAF(TM) purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse/rat MCP-1 bioactivity in vivo and in vitro (ABIN160440). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections. Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Research Area: Chemokines

Restrictions: For Research Use only