SOX2 (COOH terminus) antibody

Antigen: SOX2 (COOH terminus)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN179784

Additional Information

Immunogen: Peptide-KLH (C-terminal)

Format: Purified

Isotype: IgG  (Matching secondary antibodies)

Clone: Poly6309

Description: This rabbit polyclonal antibody recognizes human SOX2 also known as SRY (sex determining region Y)-box 2 and ANOP3. SOX2 is a nucleus protein, predicted molecular weight 34 kD. SOX2 belongs to the SOX (SRY-box containing gene) gene family which encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. Defects in SOX2 are a cause of true or primary anophthalmos, also called anophthalmia or ANOP3. Anophthalmos is a developmental defect characterized by complete absence of the eyes (rare) or by the presence of vestigial eyes. The poly6309 antibody has been shown to be useful for Western blotting. Distribution: Nucleus protein Function: The SOX gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. Synonyms: SRY (sex determining region Y)-box 2, ANOP3, SOX-2 Structure: Belongs to the SOX (SRY-box containing gene) gene family, predicted molecular weight 34 kD.

Specificity: Interacts with OCT4, OCT1, FTZ1, and Fibroblast growth factor 4 proteins.

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each minigel. For immunofluorescent staining applications, a dilution range of 1:100 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Storage: Upon receipt, store frozen at -20° C.

Restrictions: For Research Use only