Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody

Antigen: Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1)

Synonyms: KLR, CD314, NKG2D, NKG2-D, FLJ17759, FLJ75772, D12S2489E, KLRK1, Nkrp2, Nkg2d, D6H12S2489E

Clonality: Monoclonal (1D11)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN179794

Additional Information

Alternative name: NKG2D (CD314)

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: 1D11

Description: NKG2D is a homodimeric C-type lectin-like protein also known as CD314. It is expressed on NK cells, CD8 T cells, gamma/delta T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to be a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases. The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Distribution: NK cells, gamma/delta T cells, CD8 T cells Function: cytolytic killing of target cells expressing NGK2D ligands, costimulation of NK cells and T cells Synonyms: CD314 Structure: C-type lectin

Application Details

Application Notes: Additional reported (for the relevant formats) applications include: immunoprecipitation, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN179794). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 2.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Storage: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Restrictions: For Research Use only

Images

anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) antibody