IL-8 antibody

Antigen: IL-8

Clonality: Monoclonal (JK8-2)

Host: Mouse

Reactivity: Human

Application: ELISA

Catalog no.: ABIN179801

Additional Information

Immunogen: Recombinant human IL-8

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: JK8-2

Description: Neutrophil/basophil chemoattractant, activates neutrophils, angiogenic factor Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The JK8-2 antibody reacts with the 72 aa and 77 aa forms of human interleukin-8 (IL-8). Synonyms: Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8 Regulation: Upregulated by IL-1, IL-7, IL-17, IFN-gamma, TNF-alpha, leukoregulin, downregulated by IL-4, TGF-beta, glucocorticoids, vitamin D3 Structure: CXC chemokine, 8.4 kD (Mammalian)

Application Details

Application Notes: ELISA or ELISPOT Detection: The biotinylated JK8-2 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JK8-1 antibody (ABIN160567/ABIN160569) as the capture antibody. Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. Important Note: DO NOT use FBS in any solutions in this ELISA. We recommend 1% BSA for blocking and dilutions in this assay.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C.

Research Area: Cytokines, Immunology, Virology, Inflammation, Cancer

Restrictions: For Research Use only

Images

anti-IL-8 antibody