NK-1.1 antibody (APC/Cy7)

Antigen: NK-1.1

Clonality: Monoclonal (PK136)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: APC,Cy7

Application: Flow Cytometry (FACS)

Catalog no.: ABIN179805

Additional Information

Immunogen: NK-1 cells from mouse spleen and bone

Isotype: IgG2a, kappa chain

Clone: PK136

Description: NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation. Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB) Function: NK cell activation, IFN-gamma production, cytotoxic granule release Synonyms: NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c Structure: NKR-P1 gene family

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, complement-dependent cytotoxicity, in vivo depletion, mediation of in vitro redirected lysis, blocking of NK cell function, induction of proliferation, and immunohistochemistry of frozen sections and immunofluorescence microscopy. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156387). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only