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F4/80 antibody (Alexa Fluor 647)
| Antigen | F4/80 |
| Clonality | Monoclonal (CI:A3-1) |
| Host |
Rat |
| Reactivity |
Mouse (Murine) |
| Conjugate |
Alexa Fluor 647
|
| Application |
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
|
| Catalog no. | ABIN179815 |
| Quantity | 100 µg (Variants) |
| Price | Product not available in this region. |
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Additional Information
| Immunogen | Thioglycollate stimulated mouse C57/BL peritoneal macrophages |
| Isotype | IgG2b, kappa chain |
| Clone | CI:A3-1 |
| Description | F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8 T cells-mediated peripheral tolerance. Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, subset of dendritic cells Function: Induction of immunological tolerance Synonyms: EMR1, Ly71 Structure: EGF-TM7 family member, 160 kD glycoprotein |
Application Details
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections and paraffin-embedded sections, Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents. |
| Purification | The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Restrictions | For Research Use only |
