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F4/80 antibody
| Antigen | F4/80 |
| Clonality | Monoclonal (BM8) |
| Host |
Rat |
| Reactivity |
Mouse (Murine) |
| Application |
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
|
| Catalog no. | ABIN179835 |
| Quantity | 500 µg (Variants) |
| Price | Product not available in this region. |
| Shipping to |
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Additional Information
| Format | Purified |
| Isotype | IgG2a, kappa chain (Matching secondary antibodies) |
| Clone | BM8 |
| Description | F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8 T cells-mediated peripheral tolerance. Distribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, subset of dendritic cells Function: Induction of immunological tolerance Synonyms: EMR1, Ly71 Structure: EGF-TM7 family member, 160 kD glycoprotein |
Application Details
| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test. |
| Storage | The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions. |
| Restrictions | For Research Use only |
