CD45RO (CD45RO) antibody (Biotin)

Antigen: CD45RO (CD45RO)

Clonality: Monoclonal (UCHL1)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Catalog no.: ABIN179871

Additional Information

Alternative name: CD45RO

Isotype: IgG2a, kappa chain

Clone: UCHL1

Description: CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of the tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines. The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naive T cells. Distribution: Activated and memory T cells, B cell subset, monocytes, macrophages, granulocytes Function: Enhances TCR and BCR signaling Structure: Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, Western blotting, and immunoprecipitation. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

anti-CD45RO (CD45RO) antibody (Biotin)