ITGAV/ITGB3 (ITGAV/ITGB3) antibody

Antigen: ITGAV/ITGB3 (ITGAV/ITGB3)

Clonality: Monoclonal (23C6)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Antibody (Inhibition)

Catalog no.: ABIN179873

Additional Information

Alternative name: CD51/61

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: 23C6

Description: CD51/CD61 is an integrin complex known as alphaVbeta3. It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or beta3 integrin. It is a 110 kD common beta subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. The 23C6 antibody has been reported to be useful for blocking studies. Distribution: Platelets, B subset, activated T cells, monocytes/macrophages, osteoblasts, endothelial cells, melanoma Function: Adhesion, bone metabolism and apoptosis Synonyms: Vitronectin receptor, Integrin alphaV/beta3 Structure: Integrin, CD51/CD61 heterodimer, 24 kDa

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy, and blocking of cell adhesion. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN179873). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Storage: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

anti-ITGAV/ITGB3 (ITGAV/ITGB3) antibody