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Antibodies
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anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (APC)
Perforin 1 (Pore Forming Protein) (PRF1) antibody (APC)
| Antigen |
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| Synonyms |
PRF1, P1, PFP, FLH2, PFN1, HPLH2, MGC65093, Pfn, Pfp, Prf-1, Cyta, RATCYTA, MGC108712, LOC443187, perforin, prf1 |
| Clonality |
Monoclonal (DG9) |
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Host
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Reactivity
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Conjugate
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Application
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| Catalog no. |
ABIN179877 |
| Quantity |
100 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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Perforin
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Immunogen
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Purified granules from the human lymphoma cell line YT
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Isotype
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IgG2b, kappa chain
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Clone
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DG9
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Description
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Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. The dG9 antibody recognizes human and bovine perforin and has been shown to be useful for flow cytometry, immunoprecipitation, immunohistochemistry, and immunofluorescence staining. Distribution: CTL, NK (cytoplasmic granules) Function: Mediates targeted cell lysis Structure: 70 kD
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Specificity
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), does not cross-react with mouse.
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Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
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Purification
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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Research Area
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Inflammation, Immunology
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Restrictions
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For Research Use only
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Alternatives for antigen "Perforin 1 (Pore Forming Protein) (PRF1)", type "Antibodies"
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Hosts
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Mouse (21), Rabbit (8), Rat (5)
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Reactivities
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Human (23), Mouse (Murine) (8), Rat (Rattus) (3)
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Applications
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Western Blotting (WB) (17), Flow Cytometry (FACS) (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (8), Immunofluorescence (IF) (7), Immunohistochemistry (IHC) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), ELISA (Detection) (3), Enzyme Immunoassay (EIA) (3), ELISA (2)
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Conjugates
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Biotin (1), FITC (1), PE (1)
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Epitopes
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C-Term (3)
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Alternatives 
