V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog (KIT) antibody (PE/Cy7)

Antigen: V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog (KIT)

Synonyms: PBT, SCFR, C-Kit, CD117, W, Bs, Fdc, Ssm, SCO1, SCO5, SOW3, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3, c-kit, kit-A, XKrk1, KIT, MGF, LOC100221238, C-KIT

Clonality: Monoclonal (104D2)

Host: Mouse

Reactivity: Human

Conjugate: PE,Cy7

Application: Flow Cytometry (FACS)

Catalog no.: ABIN179884

Additional Information

Alternative name: CD117 (c-kit)

Immunogen: MOLM-1 megakaryocytic cell line

Isotype: IgG1, kappa chain

Clone: 104D2

Description: CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemic cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117, and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The 104D2 antibody does not block binding of c-Kit ligand. Distribution: Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML) Function: Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decrease in the number of primitive progenitor cells in mice, mutations can lead to ligand-independent receptor activation. Modifications: Multiple phosphorylation sites Synonyms: Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor Structure: Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections, immunoprecipitation, immunofluorescence microscopy. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per 10 cells in 100 µl volume or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

anti-V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog (KIT) antibody (PE/Cy7)