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Antibodies
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anti-CD34 (CD34) antibody (Alexa Fluor 647)
CD34 (CD34) antibody (Alexa Fluor 647)
| Antigen |
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| Clonality |
Monoclonal (4H11) |
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Host
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Reactivity
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Human (178), Mouse (Murine) (86), Rat (Rattus) (33), Dog (Canine) (27), Cow (Bovine) (15), Pig (Porcine) (15), Rabbit (15), Monkey (6), Primate (5), Primates (3), Rhesus Monkey (2)
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Conjugate
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FITC (34), PE (25), Biotin (16), APC (8), RPE (6), Alexa Fluor 488 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Cy5.5,PE (1), PE,DY647 (1), PE-Dyomics 647 (1), Pacific Blue (1)
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Application
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Flow Cytometry (FACS) (187), Western Blotting (WB) (86), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54), Immunofluorescence (IF) (53), Immunoprecipitation (IP) (42), Immunohistochemistry (Frozen Sections) (IHC (fro)) (37), ELISA (28), Immunohistochemistry (IHC) (13), Immunocytochemistry (ICC) (11), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1)
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| Catalog no. |
ABIN179894 |
| Quantity |
100 tests (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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CD34
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Immunogen
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human peripheral leucocytes
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Isotype
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IgG1, kappa chain
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Clone
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4H11
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Description
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CD34 is a type I monomeric sialomucin-like glycophophoprotein with the molecular weight about 105-120 kD, also know as gp105-120. It is selectively expressed on majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymic cleavage, four groups of epitopes of CD34 have been described. 4H11 antibody recognized epitope is in class III group which is resistant to sialidase/glycolyprotease and chymopapain treatment. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands. Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts Function: Cell adhesion Synonyms: gp105-120, Mucosialin, My10 Structure: 105-120 kD single chain mucin-like glycoprotein
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Application Notes
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.>BR>** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
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Purification
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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
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Storage
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The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Research Area
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CD Antigens
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Restrictions
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For Research Use only
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Alternatives for antigen "CD34 (CD34)", type "Antibodies"
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Hosts
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Mouse (140), Rabbit (55), Rat (51), Chicken (1)
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Reactivities
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Human (178), Mouse (Murine) (86), Rat (Rattus) (33), Dog (Canine) (27), Cow (Bovine) (15), Pig (Porcine) (15), Rabbit (15), Monkey (6), Primate (5), Primates (3), Rhesus Monkey (2)
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Applications
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Flow Cytometry (FACS) (187), Western Blotting (WB) (86), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54), Immunofluorescence (IF) (53), Immunoprecipitation (IP) (42), Immunohistochemistry (Frozen Sections) (IHC (fro)) (37), ELISA (28), Immunohistochemistry (IHC) (13), Immunocytochemistry (ICC) (11), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Functional Studies (Func) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1)
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Conjugates
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FITC (34), PE (25), Biotin (16), APC (8), RPE (6), Alexa Fluor 488 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Cy5.5,PE (1), PE,DY647 (1), PE-Dyomics 647 (1), Pacific Blue (1)
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Epitopes
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pTyr330 (1)
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Alternatives 
