Signal-Regulatory Protein alpha (SIRPA) antibody (Biotin)

Antigen: Signal-Regulatory Protein alpha (SIRPA)

Synonyms: BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, SHPS-1, SIRPalpha, SIRPalpha2, SIRP-ALPHA-1, SIRPA, MGC148619, SIRP-A1, SIRP-B1, SIRPB1

Clonality: Monoclonal (SE5A5)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN179936

Additional Information

Alternative name: CD172a (SIRPa)

Immunogen: NIH-3T3/hu-SIRPalpha cell line

Isotype: IgG1, kappa chain

Clone: SE5A5

Description: CD172a, kwon as signal-regulatory protein alpha (SIRPalpha), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to Ig superfamily primarily expressed on monocytes/macrophages, granulocytes, dendritic cells and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion and phagocytosis. CD47 (IAP) is an extracellular ligand for CD172a. SE5A5 antibody is against CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPalpha (SIRPalpha1 and SIRPalpha2) to CD47. Distribution: Monocytes/macrophages, granulocytes, dendritic cells, neurons Function: Negative regulation of leukocyte adhesion and transmigration, macrophage fusion, phagocytosis Synonyms: SIRPalpha, BIT, SHPS1, MFR, P84, PTPNS1 Structure: 75-110 kD glycoprotein , Signal-regulatorey-protein family, Ig superfamily

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Restrictions: For Research Use only

Images

anti-Signal-Regulatory Protein alpha (SIRPA) antibody (Biotin)