Lymphocyte Antigen 9 (LY9) antibody

Antigen: Lymphocyte Antigen 9 (LY9)

Synonyms: hly9, mLY9, CD229, SLAMF3, LY9, MGC139478, Ly-9, T100, Lgp100, AI893573

Clonality: Monoclonal (HLy-9-1-25)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN179950

Additional Information

Alternative name: CD229 (Ly-9)

Immunogen: 300.19 cell line transfected with the CD229 full-length DNA

Format: Purified

Isotype: IgG1, kappa chain

Clone: HLy-9-1-25

Description: CD229 is a 100-120 kD glycoprotein. It is a member of the SLAM family, a CD2 subset of the Ig superfamily, known as Ly9 or SLAMF3. CD229 is expressed on T cells, B cells, NK cells, and thymocytes. It functions as a homophilic adhesion molecule through binding to CD229 itself. The cytoplasmic tail of CD229 binds to SAP and Grb2 proteins. CD229 is involved in enhancing T cell activation and Th2 polarization. HLy9.1.25 antibody recognizes epitope A in the first Ig domain of Ly9 molecule. Distribution: T cells, B cells, NK cells, thymocytes Function: Homophilic adhesion, T cell activation, Th2 cell polarization Synonyms: Ly9, Ly-9, SLAMF3 Structure: 100-120 kD glycoprotein, SLAM family, CD2 subset of Ig superfamily

Application Details

Application Notes: Additional reported (for the relevant formats) applications include: immunoprecipitation, and immunocytochemistry.The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN179950). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Storage: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Research Area: CD Antigens

Restrictions: For Research Use only