IL-9 antibody
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| Clonality |
Monoclonal (D8402E8) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN180003 |
| Quantity |
500 µg (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Immunogen
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Baculovirus-expressed, recombinant mouse IL-9
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Format
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Purified
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Isotype
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IgG2a, kappa chain (Matching secondary antibodies)
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Clone
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D8402E8
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Description
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Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes, regulates granzyme family proteases, enhances proliferation of bone marrow mast cells with IL-3, production of IL-6 by mast cells IL-2 activated TH2 lymphocytes IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The D8402E8 antibody reacts with mouse IL-9. Synonyms: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3) Regulation: Upregulated by IL-1 after TCR activation, TNF-alpha, downregulated by antibodies to IL-2 Structure: Cytokine, 30-40 kD (Mammalian)
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Application Notes
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ELISA Capture: The purified D8402E8 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated D9302C12 antibody (ABIN160366) as the detecting antibody. Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
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Purification
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The antibody was purified by affinity chromatography.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
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Storage
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The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.
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Research Area
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Cytokines, Immunology, Virology, Inflammation, Cancer
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Restrictions
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For Research Use only
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Alternatives for antigen "IL-9", type "Antibodies"
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Hosts
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Rabbit (24)
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Reactivities
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Mouse (Murine) (20), Rat (Rattus) (20), Human (6), Chicken (3), Cow (Bovine) (3), Dog (Canine) (3), Pig (Porcine) (3), Horse (Equine) (1), Sheep (Ovine) (1)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (7), ELISA (6), Blocking Antibody (Inhibition) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternatives 
