IL-17 antibody
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| Clonality |
Monoclonal (TC11-8H4) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN180005 |
| Quantity |
500 µg |
| Price |
Product not available in this region.
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| Shipping to |
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Immunogen
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E. coli-expressed, recombinant mouse IL-17
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Format
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Purified
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Isotype
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IgG1, kappa chain (Matching secondary antibodies)
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Clone
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TC11-8H4
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Description
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Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells, stimulates cell migration, cord formation, and IL-6 secretion by stromal cells CD4 memory T cells IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-kappaB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-8H4 antibody antibody reacts with mouse interleukin-17 (IL-17). Synonyms: Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8) Structure: Cytokine, dimer, 15 kD (Mammalian)
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Application Notes
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ELISA and ELISPOT Detection: The biotinylated TC11-8H4 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10 antibody (ABIN160496) as the capture antibody, and with recombinant mouse IL-17 (ABIN160666) as the standard. Additional reported applications (for the relevant formats) include: Western blotting.
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Purification
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The immunoglobulin was purified by affinity chromatography.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
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Storage
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The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.
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Research Area
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Cytokines, Immunology, Virology, Inflammation, Cancer
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Restrictions
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For Research Use only
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Alternatives for antigen "IL-17", type "Antibodies"
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Hosts
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Rabbit (44), Rat (4), Mouse (2), Goat (1)
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Reactivities
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Human (28), Mouse (Murine) (24), Rat (Rattus) (19), Dog (Canine) (3), Cow (Bovine) (2), Pig (Porcine) (2), Sheep (Ovine) (2), Chicken (1), Rabbit (1)
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Applications
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Flow Cytometry (FACS) (36), Immunofluorescence (IF) (36), ELISA (15), Western Blotting (WB) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunoprecipitation (IP) (4), Blocking Antibody (Inhibition) (2), Immunoelectron Microscopy (IEM) (2), Neutralization (Neut) (1)
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Conjugates
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Biotin (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Alternatives 
