UCP4 antibody

Antigen: UCP4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN180008

Additional Information

Immunogen: Recombinant (partial), N-terminal

Format: Purified

Isotype: IgG  (Matching secondary antibodies)

Clone: Poly6161

Description: UPC4 also known as mitochondrial uncoupling protein 4 is a 36 kD member of the mitochondrial anion carrier protein family. UPC4 is expressed in mitochondria exclusively in the adult and fetal brain. UPC4 facilitates transfer of anions from the inner to the outer mitochondrial membrane and the return of protons from the outer to the inner mitochondrial membrane. This reduces mitochondrial membrane potential and induces mitochondrial leak in which oxidative phosphorylation is separated from ATP synthesis with energy dissipated as heat. UPC4 has been shown to be increased by diet, temperature, and various environmental conditions. The Poly6161 antibody recognizes the N-terminal region of UPC4 and has been shown to be useful for Western blotting. Distribution: Mitochondria. Expressed exclusively in fetal and adult brain Function: Facilitates transfer of anions from inner to outer mitochondrial membrane and the return of protons from the outer to the inner mitochondrial membrane. This reduces mitochondrial membrane potential and induces mitochondrial leak in which oxidative phosphorylation is separated from ATP synthesis with energy dissipated as heat. Synonyms: Mitochondrial uncoupling protein 4, Uncoupling protein 4 Regulation: Increased by diet, temperature, and various environmental conditions Structure: Mitochondrial anion carrier protein family (MACP), 36 kD

Application Details

Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Storage: Upon receipt, store frozen at -20° C.

Restrictions: For Research Use only