Nuclear Factor Nf-kappa-B P105 Subunit (NFKB1) antibody (FITC)

Antigen: Nuclear Factor Nf-kappa-B P105 Subunit (NFKB1)

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, p52, LYT10, LYT-10, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b

Clonality: Monoclonal (4D1)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Immunofluorescence (IF)

Catalog no.: ABIN180010

Additional Information

Alternative name: NF-kB p50

Immunogen: recombinant full-length human NF-kappaB p50

Isotype: IgG1, kappa chain

Clone: 4D1

Description: NF-kappaB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-kappaB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-kappaB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-kappaB/p50 is bound (with p65) to IkappaB inhibitor in cytoplasm in inactive form. Phosphorylation of IkappaB releases and NF-kappaB which goes to the nucleus to activate gene expression. NF-kappaB can be activated with LPS, TNF-alpha, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-kappaB p50 and has been shown to be useful for Western blotting. Distribution: Ubiquitously expressed nuclear protein Function: Transcriptional activator, originally identified as an activator of kappa light chain in B cells Synonyms: NF-kappaB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-kappaB1 Regulation: Bound (with p65) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB goes to the nucleus to activate gene expression. Stimulation with LPS, TNF-alpha, phorbol ester, or IL-1 activates Structure: Member of the Rel/dorsal family. One subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits derived from larger precursor proteins

Specificity: Interacts with NF-kappaB p65 and IkappaB.

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, p52, LYT10, LYT-10, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b

Application Details

Application Notes: For immunofluorescent staining applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: Transcription Factors

Restrictions: For Research Use only