Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) antibody (FITC)

Antigen: Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)

Synonyms: SLP76, SLP-76

Clonality: Monoclonal (H76)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN180012

Additional Information

Alternative name: SLP-76

Isotype: IgG2a, kappa chain

Clone: H76

Description: SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind, this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting. Distribution: Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung. Function: Adaptor molecule involved in B cell receptor and T cell receptor signaling Modifications: Phosphorylation (Y113, Y128, Y145, Y423, Y426) Structure: Phosphoprotein 76 kD containing SH2 domain in the C-terminus

Specificity: Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2.

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: Signaling

Restrictions: For Research Use only