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Tubulin, alpha (TUBA) antibody (Alexa Fluor 488)
| Antigen | Tubulin, alpha (TUBA) |
| Synonyms |
Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)T ...
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| Clonality | Monoclonal (10D8) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Conjugate |
Alternatives Alexa Fluor 488 |
| Application |
Alternatives Immunofluorescence (IF)
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| Catalog no. | ABIN180014 |
| Quantity | 100 µg (Variants) |
| Price | Product not available in this region. |
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Additional Information
| Alternative name | Tubulin-alpha |
| Immunogen | Recombinant full length human alpha-tubulin |
| Isotype | IgM, kappa chain |
| Clone | 10D8 |
| Description | Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes alpha-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting. Distribution: Ubiquitous Function: Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site Modifications: Phosphorylation, acetylation Synonyms: alpha-tubulin, Tubulin, alpha-ubiquitous chain Structure: Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD. |
| Specificity | RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A. |
| Synonyms | Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)Tom, Fs(3)Tomaj, Kavar, T, Tom, Tub, aTub67C, alpha-Tub, alpha-Tub67C, alpha-tubulin, alpha-tubulin[4], alpha4, alpha4-t, alpha4-tubulin, alpha4Tub67C, alpha4t, alpha67C, alphaTUB, alphaTub4, alphaTub[67C], alphatub67C, mum-tub67C, DmelCG8308, CG8308, 1t, ALPHA 84C, BEST:LD32507, DM1alpha, DTA1, Talpha1, Tuba84B, Tubalpha1, a1t, aTub84B, agr;Tub, alpha-Tub84B, alpha1, alpha1t, alpha1tub, alpha84B, alphaT, alphaTub1, alphat-1, anon-EST:Liang-1.59, anon-EST:Liang-2.30, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tubulin, DmelCG1913, CG1913, 3t, ALPHA 84D, D.m.ALPHA-84D, DTA4, aTub84D, alpha-Tub84D, alpha3, alpha3t, alpha84D, alphaTub3, DmelCG2512, CG2512, 2t, ALPHA 85E, DTA3, a2t, aTub85E, alpha-Tub85E, alpha2, alpha2t, alpha85E, alpha85E-tub, alphaTub2, alphaTub84E, DmelCG9476, CG9476, TUBA3E, TUBA2, LIS3, TUBA3, FLJ25113, B-ALPHA-1, Tuba3b, Tuba3a, RGD1565155, TUBA3D, MGC133894, tua3 |
Application Details
| Application Notes | For immunofluorescent staining applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents. |
| Purification | The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488. |
| Buffer | This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Research Area | Cytoskeleton, Cell/Tissue Markers |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "Tubulin, alpha (TUBA)", type "Antibodies"
| Hosts | Mouse (40), Rabbit (25), Rat (3) |
| Reactivities | Human (37), Mouse (Murine) (20), Rat (Rattus) (19), Chicken (8), Various Species (5), Pig (Porcine) (2), All Species (1), Yeast (Saccharomyces Cerevisiae) (1) |
| Applications | Western Blotting (WB) (44), Immunofluorescence (IF) (37), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunoprecipitation (IP) (13), ELISA (12), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (6), Immunoelectron Microscopy (IEM) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2) |
| Conjugates | Biotin (6), Alexa Fluor 488 (2), FITC (2), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Dyomics 547 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
