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Antibody
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anti-Osteopontin SPP1
Osteopontin (SPP1) antibody
| Antigen |
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| Synonyms |
OPN, BNSP, BSPI, ETA-1, MGC110940 |
| Clonality |
Monoclonal (10A16) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
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| Catalog no. |
ABIN180020 |
| Quantity |
200 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business days |
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Alternative name
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Osteopontin
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Immunogen
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Synthetic peptide for a part of Human Osteopontin
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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10A16
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Description
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Osteopontin (OPN) is a secreted glycoprotein that was originally isolated from bone. Its molecular weights have been reported in the range of 66 kDa to 44 kDa depending on glycosylation and phosphorylation. OPN is also known to be expressed in other fluids and tissues including milk, urine, activated T cells, smooth muscle cells, kidney and some tumor cells. OPN contains an Arg-Gly-Asp (RGD) amino acid sequence. This motif is present in fibronectin, vitronectin and a variety of other extra-cellular proteins that bind members of the integrin family of cell surface receptors such as a v3. Recently OPN was identified as a ligand for CD44, which levels correlate with aggressiveness of lymphoid tumors and invasiveness of bladder carcinoma. Its interaction does not require RGD motif of OPN. In OPN knockout mice, it has been reported that a significantly decreased level of debridement was shown. Although the distribution and expression pattern of OPN in the human body have suggested the multiple function of OPN, its function under different situations remain obscure.
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Specificity
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Human Osteopontin specific. No crossreactivities with mouse and rat Osteopontin.
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Application Notes
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This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 1 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of about 1 ug/ml.
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Purification
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Protein A
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 Reconstitution: 1.0 ml distilled water will be added to the product
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Storage
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Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20
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Research Area
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Cancer
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Restrictions
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For Research Use only
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Publications
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H: H: in:
Method employed by authors: H (1:H, Sample species: H). Further details: H
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Alternatives for antigen "Osteopontin (SPP1)", type "Antibodies"
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Hosts
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Mouse (42), Rabbit (26), Goat (4)
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Reactivities
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Human (64), Mouse (Murine) (13), Rat (Rattus) (11), Dog (Canine) (5), Pig (Porcine) (4)
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Applications
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Western Blotting (WB) (67), Enzyme Immunoassay (EIA) (39), ELISA (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), ELISA (Detection) (1), Flow Cytometry (FACS) (1)
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Epitopes
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AA 0-17 (3), AA 164-179 (3), AA 59-74 (3), C-Term (2)
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