ApoE4 antibody

Antigen: ApoE4

Clonality: Monoclonal (5B5)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180022

Additional Information

Immunogen: Synthetic peptide for a part of Human ApoE4

Isotype: IgG1  (Matching secondary antibodies)

Clone: 5B5

Description: Apolipoprotein-E (Apo-E) is a structural component of very low-density lipoprotein (VLDL) synthesized by the liver and intestinally synthesized chylomicrons. It is also a constituent of a subclass of high-density lipoproteins (HDLs) involved in cholesterol transport activity among cells. One of the most important roles of ApoE is to mediate high affinity binding of chylomicrons and VLDL particles that contain ApoE to the low-density lipoprotein (LDL) receptor. This allows for the specific uptake of these particles by the liver that is necessary for transport preventing the accumulation in plasma of cholesterol rich remnants. There are indications that ApoE is also involved in immune system regulation, nerve regeneration and muscle.

Specificity: Human ApoE4 specific, non-crossreactive with Human ApoE2 and ApoE3.

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after autoclave*1 (or microwave) treatment following formic acid treatment*2 by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 1-020 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of 1-0 ug /ml. *1 1-10 for 1-0 minutes (10mM citric acid buffer pH6.0) *2 Rinsing by running water after formic acid treatment for 5 minutes following de-paraffin.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only

Images

Human Brain (Alzheimer Disease)

anti-ApoE4 antibody (Image 2)

Publications

Publications: Kong, Bai, Sun et al.: "Expression of the Newly Identified Gene CAC1 in the Hippocampus of Alzheimer's Disease Patients." in: Journal of molecular neuroscience : MN, 2012 (PubMed).