Tight Junction Associated Protein antibody

Antigen: Tight Junction Associated Protein

Clonality: Monoclonal (7H6)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180031

Additional Information

Immunogen: A bile canaliculus-rich fraction prepared from rat livers

Isotype: IgM  (Matching secondary antibodies)

Clone: 7H6

Description: Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Recently, several component molecules of tight junction have been identified. This monoclonal antibody, 7H6 which reacts with a novel 155 kD tight junction-associated protein in rat livers. The 7H6 antigen is considered as an important molecule for regulation of the barrier function of tight junctions from examination in Madin-Darby canine kidney (MDCK) cells. . Expression of the 7H6 antigen was also examined during rat hepatocarcinogenesis and human cancer tissues. Semiquantitative analysis by confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially in preneoplastic lesions and cancer tissues appeared in the liver during hepatocarcinogenesis. The 7H6 antigen was expressed scarcely in HCC with a trabecular pattern, whereas it was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in expression in the poorly-differentiated adenocarcinomas. These indicate that impairment of tight junctions may be associated with malignant phenotypes of cancer cells, such as the loss of cellular polarity and adhesiveness.

Specificity: Reacts with Human

Application Details

Application Notes: This antibody can be used for immunohistochemistry (Frozen Section) in concentration of 5-20 ug/ml, and also can be used for western blotting in concentration of 0.510 ug/ml. Reference:1. Zhong Y., Saito T., Minase T., Sawada N., Enomoto K., and Mori,M. Monoclonal antibody 7H6 reacts with a novel tight junction-associated protein distinct from ZO-1, cingulin and ZO-2. J. Cell Biol. 1-20 (2): 4-77-483,1993. 2. Zhong Y., Enomoto K., Isomura H., Sawada N., Minase T., Oyamada M., Konishi Y., and Mori M. Localization of the 7H6 antigen at tight junctions correlates with the paraceilular barrie function of MDCK cells. Exp. Cell Res. 2-14: 6-14-620,1994

Purification: Anti-Mouse IgM

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only